Virus hosts i phaseolus vulgaris kidney bean french bean see also ncbi. Other viruses isolated were arabis mosaic, bean yellow mosaic, red clover mottle, and red clover vein mosaic. Independent expression of rep and repa and their roles in regulating bean yellow dwarf virus replicatio. Citrus variegation virus cvv, satsuma dwarf virus sdv, citrus leaf rugose virus clrv, and citrus yellow vein clearing virus cyvcv. Bean yellow dwarf virus replicons for highlevel transgene expression. Modifying the replication of geminiviral vectors reduces. Dsbs and doing so an insertion or deletion indel of few nucleotides takes place. Modified viral genomes of both rna and dna viruses have been created. Barley yellow dwarf virus barley yellow dwarf byd is a plant disease caused by the barley yellow dwarf virus bydv, and is the most widely distributed viral disease of cereals. A common bean plant showing symptoms of black root necrosis caused by the bean common mosaic virus bcmv. I want to tell the story of a geminivirus called bean yellow dwarf virus that. Daniel voytass lab contains the insert reprepa and is published in plant cell.
In the begomoviruses, the bean dwarf mosaic virus nuclear shuttle protein. Improvement of the transient expression system for. The cleavage gives rise to a free 3oh that serves as a primer for the cellular dna polymerase. Properties of tobacco yellow dwarf and bean summer death.
Geminiviral vectors based on bean yellow dwarf virus for. A novel stable transgenic plant expression system was developed using elements of the replication machinery of bean yellow dwarf virus beydv. A deconstructed immobilized mild strain of the bean yellow dwarf virus beydv, allowing for a high replicon copy number in the nucleus, has recently been used to construct an agrobacterium tdna that integrates into the host cell chromosome and delivers a geminivirus replicon gvr 17, 46. Bean yellow dwarf virus beydv, a mastrevirus of the geminiviridae family, contains a singlestranded circular dna genome and uses a rolling circle mechanism to replicate its genome, resulting in. It affects the economically important crop species barley, oats, wheat, maize, triticale and rice. Geminiviruses such as bean yellow dwarf virus beydv have a small, single stranded dna genome that replicates in the nucleus of an infected plant cell, using the cellular dna synthesis apparatus and a virus encoded replication initiator protein rep. To reduce crop losses due to geminivirus infection, we targeted the bean yellow dwarf virus beydv genome for destruction with the crisprcas.
Removing nonessential features of the virus also gives more room for. Bean yellow dwarf virus beydv, a mastrevirus of the geminiviridae family, contains a singlestranded circular dna genome and uses a rolling circle mechanism to. Viral vectors for plant genome engineering europe pmc article. Leaves of a common bean plant showing symptoms of primary leaf necrosis caused by the bean common mosaic virus bcmv. Amplification of genomeintegrated beydv replicons by transient. In addition to producing similar host reactions and having similar death virus bsdv, contained viruslike particles occurring in pairs about vector relations, tydv and bsdv are serologically related, and are 20 nm x 35 nm in size. Geminiviruses such as bean yellow dwarf virus beydv have a small, single. Exceptions to the rule are tydv and bean yellow dwarf virus beydv, which infect. Conferring resistance to geminiviruses with the crisprcas. We find that a modest reduction in expression of rep and repa reduces. Raspberry yellow dwarf, a soilborne virus request pdf. Bean yellow dwarf virus putative genes v1, v2, c1, c1.
Pdf geminiviral vectors based on bean yellow dwarf virus for. Nutrient deficient yellowing leaves bean fix this week. Bean diseases and pests, description, uses, propagation. After one round of replication, a repcatalyzed nucleotidyl transfer reaction releases a circular singlestranded virus genome, thereby terminating the replication. The replicon dna band intensity was quantified using imagej software, using the. C1c2 replicationassociated protein bean yellow dwarf. This week in the garden ut extension agent chris cooper takes a look at some bush beans with yellowing leaves.